Search scope:
排序: Display mode:
Transcription Factors HNF1A, HNF4A, and FOXA2 Regulate Hepatic Cell Protein N-Glycosylation Article
Vedrana Vičić Bočkor,Nika Foglar,Goran Josipović,Marija Klasić,Ana Vujić,Branimir Plavša,Toma Keser,Samira Smajlović,Aleksandar Vojta,Vlatka Zoldoš
Engineering 2024, Volume 32, Issue 1, Pages 58-69 doi: 10.1016/j.eng.2023.09.019
Keywords: Clustered regularly interspaced short palindromic repeats/dead Cas9 (CRISPR/dCas9) Epigenetics Hepatocyte
Hongyuan Zhang, Yifei Zhang, Wei-Xiao Wang, Weizhong Chen, Xia Zhang, Xingxu Huang, Wei Chen, Quanjiang Ji
Engineering 2022, Volume 15, Issue 8, Pages 67-77 doi: 10.1016/j.eng.2022.02.013
Keywords: CRISPR Cas9 Mycobacterium tuberculosis Genome editing Base editing
Mobile CRISPR-Cas9 based anti-phage system in E. coli
Frontiers of Chemical Science and Engineering 2022, Volume 16, Issue 8, Pages 1281-1289 doi: 10.1007/s11705-022-2141-7
Keywords: phage infections anti-phage CRISPR-Cas9 conjugative transfer synthetic biology
Mengxu GE,Fei LIU,Fei CHANG,Zhaolin SUN,Jing FEI,Ying GUO,Yunping DAI,Zhengquan YU,Yaofeng ZHAO,Ning LI,Qingyong MENG
Frontiers of Agricultural Science and Engineering 2015, Volume 2, Issue 3, Pages 242-248 doi: 10.15302/J-FASE-2015059
Keywords: lactoferrin promoter CRISPR/Cas9 plasmid pX330
One-step generation of myostatin gene knockout sheep via the CRISPR/Cas9 system
Hongbing HAN,Yonghe MA,Tao WANG,Ling LIAN,Xiuzhi TIAN,Rui HU,Shoulong DENG,Kongpan LI,Feng WANG,Ning LI,Guoshi LIU,Yaofeng ZHAO,Zhengxing LIAN
Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 1, Pages 2-5 doi: 10.15302/J-FASE-2014007
CRISPR-Cas9 mediated LAG-3 disruption in CAR-T cells
Yongping Zhang, Xingying Zhang, Chen Cheng, Wei Mu, Xiaojuan Liu, Na Li, Xiaofei Wei, Xiang Liu, Changqing Xia, Haoyi Wang
Frontiers of Medicine 2017, Volume 11, Issue 4, Pages 554-562 doi: 10.1007/s11684-017-0543-6
Keywords: CAR-T CRISPR-Cas9 LAG-3
Embryo-mediated genome editing for accelerated genetic improvement of livestock
Zachariah MCLEAN, Björn OBACK, Götz LAIBLE
Frontiers of Agricultural Science and Engineering 2020, Volume 7, Issue 2, Pages 148-160 doi: 10.15302/J-FASE-2019305
Selecting beneficial DNA variants is the main goal of animal breeding. However, this process is inherently inefficient because each animal only carries a fraction of all desirable variants. Genome editing technology with its ability to directly introduce beneficial sequence variants offers new opportunities to modernize animal breeding by overcoming this biological limitation and accelerating genetic gains. To realize rapid genetic gain, precise edits need to be introduced into genomically-selected embryos, which minimizes the genetic lag. However, embryo-mediated precision editing by homology-directed repair (HDR) mechanisms is currently an inefficient process that often produces mosaic embryos and greatly limits the numbers of available edited embryos. This review provides a summary of genome editing in bovine embryos and proposes an embryo-mediated accelerated breeding scheme that overcomes the present efficiency limitations of HDR editing in bovine embryos. It integrates embryo-based genomic selection with precise multi-editing and uses embryonic cloning with elite edited blastomeres or embryonic pluripotent stem cells to resolve mosaicism, enable multiplex editing and multiply rare elite genotypes. Such a breeding strategy would enable a more targeted, accelerated approach for livestock improvement that allows stacking of beneficial variants, even including novel traits from outside the breeding population, in the most recent elite genetic background, essentially within a single generation.
Keywords: animal breeding cattle cloning CRISPR/Cas9 cytoplasmic injection embryo genome editing germline chimaeras
is essential for the integrity of stereociliary rootlet in cochlear hair cells in mice
Yuqin Men, Xiujuan Li, Hailong Tu, Aizhen Zhang, Xiaolong Fu, Zhishuo Wang, Yecheng Jin, Congzhe Hou, Tingting Zhang, Sen Zhang, Yichen Zhou, Boqin Li, Jianfeng Li, Xiaoyang Sun, Haibo Wang, Jiangang Gao
Frontiers of Medicine 2019, Volume 13, Issue 6, Pages 690-704 doi: 10.1007/s11684-018-0638-8
Keywords: TPRN stereocilia stereociliary rootlet actin filament CRISPR/Cas9 hearing
Acetylated HOXB9 at lysine 27 is of differential diagnostic value in patients with pancreatic ductal
Xiaoran Sun, Jiagui Song, Jing Zhang, Jun Zhan, Weigang Fang, Hongquan Zhang
Frontiers of Medicine 2020, Volume 14, Issue 1, Pages 91-100 doi: 10.1007/s11684-019-0696-6
Keywords: HOXB9 AcK27-HOXB9 PDAC
662 A/G gene variation in human tumor necrosis factor receptor superfamily, member 9 (TNFRSF9)
QU Yanchun, YANG Ze, SUN Liang, JI Linong
Frontiers of Medicine 2008, Volume 2, Issue 3, Pages 283-285 doi: 10.1007/s11684-008-0053-7
Keywords: D-HPLC mutation development autoimmune PCR-RFLP candidate
The 9th China Engineering Management Forum Guangzhou Consensus
Frontiers of Engineering Management 2015, Volume 2, Issue 2, Pages 105-107 doi: 10.15302/J-FEM-2015019
REDUCTION OF NON-POINT SOURCE POLLUTION IN THE YONG’AN RIVER BY CONSTRUCTED WETLAND BASED ON 9 YEARS
Frontiers of Agricultural Science and Engineering 2023, Volume 10, Issue 4, Pages 627-638 doi: 10.15302/J-FASE-2023516
Keywords: inflowing rivers surface-flow constructed wetland nutrients long-term monitoring
Construction of lentiviral vector carrying Rab9 gene and its expression in mouse brain
Youguo HAO, Min ZHANG, Jinzhi XU, Bitao BU, Jiajun WEI
Frontiers of Medicine 2009, Volume 3, Issue 2, Pages 141-147 doi: 10.1007/s11684-009-0041-6
Keywords: Rab9 lentivirus gene therapy gene transfer
The measurement and analysis of ice induced vibration of JZ9-3WHPB oil platform in Bohai Sea
lan Zhigang,Zhao Yinnina,Guo Jianfeng,Zhou Zhentao,Xing Jian,Bai Gang,Lu Hongge,Li Huihui
Strategic Study of CAE 2011, Volume 13, Issue 5, Pages 79-87
Based on the structure and layout of the untended JZ9-3WHPB platform,remote controllable ice induced vibration monitoring system was designed and installed on JZ9-3WHPBShort time Fourier transform was employed to study the temporal variations and mode frequencies of the
Keywords: ffshore platform sea ice of Bohai Sea ice induced vibration vibration acceleration short time Fourier
Compiling of comprehensive data of human infections with novel influenza A (H7N9) virus
Ying Wu, George F. Gao
Frontiers of Medicine 2013, Volume 7, Issue 3, Pages 275-276 doi: 10.1007/s11684-013-0285-z
Title Author Date Type Operation
Transcription Factors HNF1A, HNF4A, and FOXA2 Regulate Hepatic Cell Protein N-Glycosylation
Vedrana Vičić Bočkor,Nika Foglar,Goran Josipović,Marija Klasić,Ana Vujić,Branimir Plavša,Toma Keser,Samira Smajlović,Aleksandar Vojta,Vlatka Zoldoš
Journal Article
PAM-Expanded Streptococcus thermophilus Cas9 C-to-T and C-to-G Base Editors for Programmable Base Editing
Hongyuan Zhang, Yifei Zhang, Wei-Xiao Wang, Weizhong Chen, Xia Zhang, Xingxu Huang, Wei Chen, Quanjiang Ji
Journal Article
Generation of CRISPR/Cas9-mediated lactoferrin-targeted mice by pronuclear injection of plasmid pX330
Mengxu GE,Fei LIU,Fei CHANG,Zhaolin SUN,Jing FEI,Ying GUO,Yunping DAI,Zhengquan YU,Yaofeng ZHAO,Ning LI,Qingyong MENG
Journal Article
One-step generation of myostatin gene knockout sheep via the CRISPR/Cas9 system
Hongbing HAN,Yonghe MA,Tao WANG,Ling LIAN,Xiuzhi TIAN,Rui HU,Shoulong DENG,Kongpan LI,Feng WANG,Ning LI,Guoshi LIU,Yaofeng ZHAO,Zhengxing LIAN
Journal Article
CRISPR-Cas9 mediated LAG-3 disruption in CAR-T cells
Yongping Zhang, Xingying Zhang, Chen Cheng, Wei Mu, Xiaojuan Liu, Na Li, Xiaofei Wei, Xiang Liu, Changqing Xia, Haoyi Wang
Journal Article
Embryo-mediated genome editing for accelerated genetic improvement of livestock
Zachariah MCLEAN, Björn OBACK, Götz LAIBLE
Journal Article
is essential for the integrity of stereociliary rootlet in cochlear hair cells in mice
Yuqin Men, Xiujuan Li, Hailong Tu, Aizhen Zhang, Xiaolong Fu, Zhishuo Wang, Yecheng Jin, Congzhe Hou, Tingting Zhang, Sen Zhang, Yichen Zhou, Boqin Li, Jianfeng Li, Xiaoyang Sun, Haibo Wang, Jiangang Gao
Journal Article
Acetylated HOXB9 at lysine 27 is of differential diagnostic value in patients with pancreatic ductal
Xiaoran Sun, Jiagui Song, Jing Zhang, Jun Zhan, Weigang Fang, Hongquan Zhang
Journal Article
662 A/G gene variation in human tumor necrosis factor receptor superfamily, member 9 (TNFRSF9)
QU Yanchun, YANG Ze, SUN Liang, JI Linong
Journal Article
REDUCTION OF NON-POINT SOURCE POLLUTION IN THE YONG’AN RIVER BY CONSTRUCTED WETLAND BASED ON 9 YEARS
Journal Article
Construction of lentiviral vector carrying Rab9 gene and its expression in mouse brain
Youguo HAO, Min ZHANG, Jinzhi XU, Bitao BU, Jiajun WEI
Journal Article
The measurement and analysis of ice induced vibration of JZ9-3WHPB oil platform in Bohai Sea
lan Zhigang,Zhao Yinnina,Guo Jianfeng,Zhou Zhentao,Xing Jian,Bai Gang,Lu Hongge,Li Huihui
Journal Article